20+ Colony Formation Assay Crystal Violet Background. Before beginning a colony formation assay, the dose of antibiotic required to kill your target cell line needs to be empirically determined. Tips are provided throughout the video to help optimize the assay procedure, including tricks to accurately evaluate and score colony formations.
Colony forming assay. (A) Colony forming assay of primary ... from www.researchgate.net
The colony formation assay evaluates the proliferative capacity of a single cell. Remove media o add 1 ml of pbs containing 4% formaldehyde and 0.005% crystal violet to each well (mix 19.9ml pbs + 100 µl 100x crystal violet stain). 0.1% crystal violet 0.22 μm polyethersulfone filter (for crystal violet solution)
The clonogenic (or colony forming) assay has been established for more than 50 years;
Stain the colonies with crystal violet and count them if so desired. Divide plates into quarters to help with high counts. Place small water bath in the tc hood. Staining colonies with crystal violet.
