29+ Colony Pcr Plate Pics. Using a pipette tip or a sterile toothpick, pick individual colonies from the transformation plates, and transfer them to their respective. Setting up colony pcr reactions is nearly identical to preparing a standard pcr reaction:
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Km is an abbreviation for kanamicin resistance.
In 1985, polymerase chain reaction, or pcr, was invented by kary mullis. Label at least 8 colonies on a plate with a marker (circle them and assign a number) pick and dissolve run the pcr product on an agarose gel and check the result pick the good colony from the plate and let. Add 1ál of platinum taq polymerase diluted 1:2 in 1x colony pcr buffer. Our pcr machine takes 24 tubes so i routinely screen 22 colonies + 1 negative + 1 positive control.
